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Characterization of phosphopeptide enrichment strategy for mass spectrometry-based proteomic analysis

ABSTRACT: We attempted to evaluate four strategies for phosphopeptide enrichment, using the titanium dioxide (TiO2) and Phos-tag ligand particles, from in-solution or in-gel digests prior to mass spectrometry-based proteomic analysis.

ORGANISM(S): Homo Sapiens (human) Cellular Organisms

SUBMITTER: Yayoi Kimura

PROVIDER: PXD026295 | JPOST Repository | Fri Dec 24 00:00:00 GMT 2021

REPOSITORIES: jPOST

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			Action	DRS
	180912QE_OVA_aga_1.raw	Raw
	180912QE_OVA_aga_2.raw	Raw
	180912QE_OVA_aga_3.raw	Raw
	180912QE_OVA_mag_1.raw	Raw
	180912QE_OVA_mag_2.raw	Raw

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Evaluation of four phosphopeptide enrichment strategies for mass spectrometry-based proteomic analysis.

Ino Yoko Y Kinoshita Eiji E Kinoshita-Kikuta Emiko E Akiyama Tomoko T Nakai Yusuke Y Nishino Kohei K Osada Makoto M Ryo Akihide A Hirano Hisashi H Koike Tohru T Kimura Yayoi Y

Proteomics 20211228 7

Information about phosphorylation status can be used to prioritize and characterize biological processes in the cell. Various analytical strategies have been proposed to address the complexity of phosphorylation status and comprehensively identify phosphopeptides. In this study, we evaluated four strategies for phosphopeptide enrichment, using titanium dioxide (TiO<sub>2</sub> ) and Phos-tag ligand particles from in-gel or in-solution digests prior to mass spectrometry-based analysis. Using TiO< ...[more]

PMID: 34932266

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Inflammatory response to titanium dioxide particles exposure is enhanced during pregnancy

Project description:Rationale: Maternal immune responses can promote allergy development in offspring. Pilot data show that neonates of mother mice exposed during pregnancy to air pollution particles have increased allergic susceptibility. Objective: We investigated whether inflammatory response to titanium dioxide (TiO2) particles earlier considered immunologically ‘inert’ is enhanced during pregnancy. Methods: Pregnant BALB/c mice (or non-pregnant controls) received particle suspensions intranasally at day 14 of pregnancy. Lung inflammatory responses were evaluated 19 and 48 h after exposure. Results: Pregnant mice showed robust and persistent acute inflammatory responses after exposure to TiO2, while non-pregnant females had the expected minimal responses. Genomic profiling identified genes differentially expressed in pregnant lungs exposed to TiO2. Neonates of mothers exposed to TiO2 (but not PBS) developed increased susceptibility to allergens. Conclusion: Pregnancy enhances lung inflammatory responses to otherwise relatively innocuous inert particles. Keywords: Particles exposure, pregnancy vs normal

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2014-07-17 | GSE59470 | GEO

Titanium dioxide nanoparticles exposure

Project description:This project utilized oligonucleotide microarrays to examine gene expression patterns in adult male fathead minnows (Pimephales promelas) exposed to a common nanomaterial, titanium dioxide (TiO2, stearate-coated particles, MT-100TV®). We exposed adult male fathead minnows for 96 hr to three doses (0, 1, and 10 mg/L nominal) of TiO2 under static renewal conditions. TiO2 is stearate coated, 15 nm particle size. Three-condition experiment: high dose, controls (untreated, low dose). Three tissues: liver, gill, brain. Biological replicates: 4 control replicates, 4 low dose, 4 high dose for gill and liver. One array for brain control and low dose; and 2 arrays for brain high dose. Contributor: Johnson & Johnson Worldwide Envrionmental

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An Adaptive Response through N6-Methyladenosine in Fetal Offspring following Gestational Nano-TiO2 Inhalation Exposure I

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Project description:Acute phase reactants serum amyloid A-1, 3 and micro RNA-135b, -449a, and -1 are induced in lungs of mice exposed to subtoxic doses of nano-titanium dioxide particles by inhalation In the present study we investigate pulmonary mRNA and miRNA profiles of mice exposed to subtoxic dose of nano-titanium dioxide particles by inhalation. We show dramatic induction of acute phase reactants, chemoattractants, immune and host defence related genes. We also demonstrate for the first time changes in miRNA profiles in the lungs in response to nanoTiO2. Keywords: Toxicology, disease state analysis, biomarkers of health effects

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