Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Identification of TUBL interactors in HaCaT

ABSTRACT: FLAG-tagged human TUBL or human TUBL were transiently expressed in HaCaT, and then lysis and immunoprecipitation with agarose beads-conjugated antibodies to FLAG. The bound protein were eluted from the beads with FLAG peptide.

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Keiichi I. Nakayama

PROVIDER: PXD026388 | JPOST Repository | Tue Jun 01 00:00:00 GMT+01:00 2021

REPOSITORIES: jPOST

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	Control_1-1.raw	Raw
	Control_1-2.raw	Raw
	Control_1-3.raw	Raw
	Control_1-4.raw	Raw
	Control_1-5.raw	Raw

Items per page:

1 - 5 of 20

Similar Datasets

Identification of TUBL interactors in Normal Human epidermal keratinocyte (NHEK)

Project description:FLAG-tagged human TUBL or human TUBL were transiently expressed in NHEK, and then lysis and immunoprecipitation with agarose beads-conjugated antibodies to FLAG. The bound protein were eluted from the beads with FLAG peptide.

2021-06-01 | PXD026389 | JPOST Repository

HST2 interactors and HST2 phosphorylation

Project description:From yeast cells expressing Flag-Hst2, soluble (cytosolic) fractions were collected. Cells expressing untagged Hst2 served as control. IP was performed on both experimantal and conrol samples with Anti-Flag agarose beads. Flag-Hst2 and bound proteins were precipitated und purified running a short SDS-gel. LFQ was used to identify binding partners and search for S, T, and Y phosphorylation were performed separately.

2020-10-14 | MSV000086283 | MassIVE

Mass spectrometry analysis of USP38 interactors

Project description:After ectopic expression of Flag-USP38 or an empty vector in HEK293T cells for 48 hours, cells were lysed, and the supernatant after lysis was added to magnetic beads coupled with Flag antibodies. The mixture was incubated at 4°C overnight for pull-down. Subsequently, the magnetic beads were washed three times with lysis buffer and once with PBS. The magnetic bead pellet was retained for mass spectrometry identification and analysis.

| PXD055320 |

overexpression flag-DYRK1A in HEK293T

Project description:supernatants were subjected to immunoprecipitation using the appropriate antibodies conjugated to protein A/G-agarose beads overnight.

2024-11-12 | PXD057773 |

Phosphorylation sites on human Bora were identified by mass spectrometry

Project description:HEK-293T cells transiently expressing Flag-Bora were subjected to immunoprecipitation (IP) using Flag-tag antibody-conjugated beads, followed by mass spectrometry.

| PXD066630 |

Identification of the BRD1 interactome and its impact on schizophrenia risk

Project description:We identified protein-protein interactions and chromatin binding sites for two isforms of BRD1 (BRD1-S and BRD1-L) using Co-IP MS/MS and ChIP-seq. BRD1 isoforms were cloned, epitope-tagged (pcDNA 6 V5-His6, Lifetechnologies) and stably expressed in HEK293T cells. Chromatin immunoprecipitations were performed using anti V5-antibody conjugated agarose beads (Sigma-Aldrich) and anti HA antibody conjugated agarose beads (Sigma-Aldrich) as controls

2015-10-29 | E-GEOD-62811 | biostudies-arrayexpress

MS phosphor-sites of FSP1-Flag IP sample

Project description:FSP1-Flag was immunoprecipitated using Anti-DYKDDDDK-Tag mAb agarose conjugated beads (Abmart, M20018), then eluted with 3X Flag Peptide. The phosphorylated FSP1 proteins were subjected to MS analysis.

| PXD045977 |

Immunoprecipitation of mitochondrial ribosome complex of Toxoplasma gondii

Project description:Mitochondrial ribosomes in Apicomplexan parasite Toxoplasma gondii are expected to be divergent from all the other known mitoribosomes owing to the extremely fragmented and reduced rRNAs. To elucidate the structure and composition of these mitoribosomes, we performed immunoprecipitation of the FLAG epitope tagged bL12m subunit of mitoribosome using anti-FLAG M2 agarose beads. The elute was used to freeze CryoEM grids for single particle analysis and bound beads were subjected to Liquid chromatography–mass spectrometry (LC–MS) to analyse the co-immunoprecipitated proteins.

2025-04-21 | PXD053944 | Pride

LIN-41 RIP-Seq

Project description:FLAG-GFP tagged LIN-41 was immunoprecipitated using FLAG dynabeads (Sigma) from lysates of young adult C.elegans. After washing steps, bound fraction (IP) was eluted from beads and associated RNAs were isolated by using the Pico pure RNA isloation kit (Invitrogen)

2018-04-25 | GSE106814 | GEO

NUPR1 inhibits hyperPARylation-associated cell death

Project description:Nuclear Protein 1 (NUPR1) is a nuclear intrinsically disordered protein of 82 amino acids that plays an important role in stress response and in cancer development. The project was aimed at identifying binding partners of NUPR1 in MiaPaCa-2 cells, in control and after stress condition: glucose starvation during 24h. Lysates from MiaPaCa-2 cells (transfected with NUPR1-Flag or NUPR1-GFP) were used for immunoprecipitation, performed with agarose beads coated with anti-Flag or anti-GFP antibodies. After immunoprecipitation, the eluted proteins were analyzed by LC-MS/MS.

2022-07-29 | PXD035236 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data