Dataset Information

MCF7 xenografts with 5-FU in vivo ITDR-CETSA

ABSTRACT: MCF7 xenograft-bearing mice were treated intravenously for 22h with either vehicle or different 5-FU doses (10, 50, 100, and 150 mg/kg). Each xenograft was cut in similar-sized pieces and assembled into an ITDRCETSA scheme with 2 different heating temperatures (37ºC, 52ºC) and 3 xenograft pieces for each temperature. The ITDRCETSA samples were labelled using TMT10-plex. The labelling order of samples in the TMT10 channels for was as follows: Tumour pieces 1, 2, and 3: Vehicle_52C (126), 10mg/kg_52C (127N), 50mg/kg_52C (127C), 100mg/kg_52C (128N), 150mg/kg_52C (128C), Vehicle_37C (129N), 10mg/kg_37C (129C), 50mg/kg_37C (130N), 100mg/kg_37C (130C), 150mg/kg_37C (131). For additional details see the methods section of the associated publication.

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Pär Nordlund

PROVIDER: PXD026470 | JPOST Repository | Thu Jul 15 00:00:00 BST 2021

REPOSITORIES: jPOST

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Dataset's files

Source:

			Action	DRS
	MCF7_xenografts_5FU_TP1_FR1.raw	Raw
	MCF7_xenografts_5FU_TP1_FR2.raw	Raw
	MCF7_xenografts_5FU_TP2_FR2.raw	Raw
	MCF7_xenografts_5FU_TP2_FR3.raw	Raw
	MCF7_xenografts_5FU_TP2_FR4.raw	Raw

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Publications

CETSA interaction proteomics define specific RNA-modification pathways as key components of fluorouracil-based cancer drug cytotoxicity.

Liang Ying Yu YY Bacanu Smaranda S Sreekumar Lekshmy L Ramos Anderson Daniel AD Dai Lingyun L Michaelis Martin M Cinatl Jindrich J Seki Takahiro T Cao Yihai Y Coffill Cynthia R CR Lane David P DP Prabhu Nayana N Nordlund Pär P

Cell chemical biology 20210716 4

The optimal use of many cancer drugs is hampered by a lack of detailed understanding of their mechanism of action (MoA). Here, we apply a high-resolution implementation of the proteome-wide cellular thermal shift assay (CETSA) to follow protein interaction changes induced by the antimetabolite 5-fluorouracil (5-FU) and related nucleosides. We confirm anticipated effects on the known main target, thymidylate synthase (TYMS), and enzymes in pyrimidine metabolism and DNA damage pathways. However, m ...[more]

PMID: 34265272

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