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Phosphorylation-dependent Regnase-1 interactome

ABSTRACT: In this study, we exploited interactome-based approach to isolate Regnase-1 protein complexes and found that TLR-ligand, IL-1β, or IL-17 stimulation induces formation of the Regnase-1-14-3-3 or -βTRCP complex in a mutually exclusive manner.

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Osamu Takeuchi

PROVIDER: PXD026561 | JPOST Repository | Tue Oct 12 00:00:00 BST 2021

REPOSITORIES: jPOST

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IRAK1-dependent Regnase-1-14-3-3 complex formation controls Regnase-1-mediated mRNA decay.

Akaki Kotaro K Ogata Kosuke K Yamauchi Yuhei Y Iwai Noriki N Tse Ka Man KM Hia Fabian F Mochizuki Atsushi A Ishihama Yasushi Y Mino Takashi T Takeuchi Osamu O

eLife 20211012

Regnase-1 is an endoribonuclease crucial for controlling inflammation by degrading mRNAs encoding cytokines and inflammatory mediators in mammals. However, it is unclear how Regnase-1-mediated mRNA decay is controlled in interleukin (IL)-1β- or Toll-like receptor (TLR) ligand-stimulated cells. Here, by analyzing the Regnase-1 interactome, we found that IL-1β or TLR stimulus dynamically induced the formation of Regnase-1-β-transducin repeat-containing protein (βTRCP) complex. Importantly, we also ...[more]

PMID: 34636324

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