Papi interacting proteins in mitochondrial fraction of BmN4 cells

ABSTRACT: We incubated Papi-Flag in BmN4 mitochondrial lysates in the presence of 32P-ATP. Upon incubation, Papi-Flag was 32P-labeled, suggesting the presence of kinases for Papi in the fraction. Papi-Flag was immunoprecipitated after incubation in mitochondrial lysates under ATP-free conditions. The proteins were eluted from the beads with a high salt buffer and subjected to LC-MS/MS. This identified six proteins of which were annotated as kinases.

ORGANISM(S): Bombyx Mori

SUBMITTER: Mikiko C. Siomi 

PROVIDER: PXD027396 | JPOST Repository | Tue Feb 01 00:00:00 GMT 2022

REPOSITORIES: jPOST