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Characterization of HLA-A*02:01 MHC Immunopeptide Antigens Enhanced by Ultraviolet Photodissociation Mass Spectrometry

ABSTRACT: Triplicate results for 193 nm UVPD, HCD, and EThcD of a mixture of 157 synthetic peptide mixture, and 193 nm UVPD, HCD, and EThcD of BB7.2 and W6/32 immunoprecipitations

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Jennifer Brodbelt

PROVIDER: PXD027830 | JPOST Repository | Fri Aug 13 00:00:00 BST 2021

REPOSITORIES: jPOST

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Characterization of HLA-A*02:01 MHC Immunopeptide Antigens Enhanced by Ultraviolet Photodissociation Mass Spectrometry.

Watts Eleanor E Potts Gregory K GK Ready Damien B DB George Thompson Alayna M AM Lee Janice J Escobar Edwin E EE Patterson Melanie J MJ Brodbelt Jennifer S JS

Analytical chemistry 20210923 39

Identifying major histocompatibility complex (MHC) class I immunopeptide antigens represents a key step in the development of immune-based targeted therapeutics and vaccines. However, the complete characterization of these antigens by tandem mass spectrometry remains challenging due to their short sequence length, high degree of hydrophobicity, and/or lack of sufficiently basic amino acids. This study seeks to address the potential for 193 nm ultraviolet photodissociation (UVPD) to improve the a ...[more]

PMID: 34553926

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