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Phosphoproteomics of nucleocapsid protein of SARS-CoV-2

ABSTRACT: We aimed to identify the functional phosphorylation of SARS-CoV-2 nucleoprotein. For this purpose, we used the Phos-tag technology to purify and enrich virus-derived phosphopeptides.

ORGANISM(S): Cellular Organisms

SUBMITTER: Yayoi Kimura

PROVIDER: PXD027939 | JPOST Repository | Fri Jan 28 00:00:00 GMT 2022

REPOSITORIES: jPOST

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			Action	DRS
	210323Elite_Ingel_cont_1_2_6ul.csv	Csv
	210323Elite_Ingel_cont_1_2_6ul.mgf	Mgf
	210323Elite_Ingel_cont_1_2_6ul.raw	Raw
	210323Elite_Ingel_cont_3_4_6ul.csv	Csv
	210323Elite_Ingel_cont_3_4_6ul.mgf	Mgf

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Publications

Phosphopeptide enrichment using Phos-tag technology reveals functional phosphorylation of the nucleocapsid protein of SARS-CoV-2.

Ino Yoko Y Nishi Mayuko M Yamaoka Yutaro Y Miyakawa Kei K Jeremiah Sundararaj Stanleyraj SS Osada Makoto M Kimura Yayoi Y Ryo Akihide A

Journal of proteomics 20220129

Phosphorylation of viral proteins serves as a regulatory mechanism during the intracellular life cycle of infected viruses. There is therefore a pressing need to develop a method to efficiently purify and enrich phosphopeptides derived from viral particles in biological samples. In this study, we utilized Phos-tag technology to analyze the functional phosphorylation of the nucleocapsid protein (N protein; NP) of severe respiratory syndrome coronavirus 2 (SARS-CoV-2). Viral particles were collect ...[more]

PMID: 35093569

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