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A stress-reduced passaging technique improves the viability of human pluripotent cells

ABSTRACT: Xeno-free culture systems have expanded the application of human pluripotent stem cells (PSCs). Here we describe an improved method for the subculture of human PSCs. The revised method significantly facilitated the viability of human PSCs by lowering DNA damage and apoptosis, resulting in more efficient and reproducible downstream applications. Furthermore, the method did not alter the PSC characteristics after long-term culture and attenuated the growth advantage of abnormal subpopulations. This robust passaging method minimizes experimental error and improves the quality control of human PSC research and applications.

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Mio Iwasaki 

PROVIDER: PXD028731 | JPOST Repository | Sun Jan 23 00:00:00 GMT 2022

REPOSITORIES: jPOST

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A stress-reduced passaging technique improves the viability of human pluripotent cells.

Takahashi Kazutoshi K   Okubo Chikako C   Nakamura Michiko M   Iwasaki Mio M   Kawahara Yuka Y   Tabata Tsuyoshi T   Miyamoto Yousuke Y   Woltjen Knut K   Yamanaka Shinya S  

Cell reports methods 20220124 2

Xeno-free culture systems have expanded the clinical and industrial application of human pluripotent stem cells (PSCs). However, reproducibility issues, often arising from variability during passaging steps, remain. Here, we describe an improved method for the subculture of human PSCs. The revised method significantly enhances the viability of human PSCs by lowering DNA damage and apoptosis, resulting in more efficient and reproducible downstream applications such as gene editing and directed di  ...[more]