Proteomics

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LFQ of biotinylated peptides from HEK293T cells expressing AirID-CRBN


ABSTRACT: HEK293T cells stably expressing AGIA-AirID-CRBN-WT were treated with DMSO, 20 µM thalidomide, 10 µM lenalidomide, 10 µM pomalidomide, or 20 µM 5-hydroxythalidomide (5HT) in the presence of 10 µM biotin and 5 µM MG132 for 8 h in three biological replicates. After tryptic digestion, biotinylated peptides were enriched with Tamavidin 2-REV and analyzed by LC-MS/MS.

ORGANISM(S): Homo sapiens  

SUBMITTER: Hidetaka Kosako 

PROVIDER: PXD028756 | JPOST Repository | Sat Nov 06 00:00:00 GMT 2021

REPOSITORIES: jPOST

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