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LFQ of indisulam-dependent biotinylated peptides from HCT116 cells expressing AirID-DCAF15

ABSTRACT: HCT116 cells stably expressing AGIA-AirID-DCAF15-WT or -D475N mutant were treated with DMSO or 5 µM indisulam in the presence of 50 µM biotin and 10 µM MG132 for 6 h in three biological replicates. After tryptic digestion, biotinylated peptides were enriched with Tamavidin 2-REV and analyzed by LC-MS/MS.

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Hidetaka Kosako

PROVIDER: PXD028762 | JPOST Repository | Sat Nov 06 00:00:00 GMT 2021

REPOSITORIES: jPOST

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A proximity biotinylation-based approach to identify protein-E3 ligase interactions induced by PROTACs and molecular glues.

Yamanaka Satoshi S Horiuchi Yuto Y Matsuoka Saya S Kido Kohki K Nishino Kohei K Maeno Mayaka M Shibata Norio N Kosako Hidetaka H Sawasaki Tatsuya T

Nature communications 20220110 1

Proteolysis-targeting chimaeras (PROTACs) as well as molecular glues such as immunomodulatory drugs (IMiDs) and indisulam are drugs that induce interactions between substrate proteins and an E3 ubiquitin ligases for targeted protein degradation. Here, we develop a workflow based on proximity-dependent biotinylation by AirID to identify drug-induced neo-substrates of the E3 ligase cereblon (CRBN). Using AirID-CRBN, we detect IMiD-dependent biotinylation of CRBN neo-substrates in vitro and identif ...[more]

PMID: 35013300

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Neo-substrate selectivity of IMiDs on biotinylation by AirID-CRBN

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Next Generation DNA sequencing of telomere fusion events isolated from HCT116 LIG3-/-:mL3, HCT116 LIG4-/-, WT HCT116 and MRC5HPVE6E7 cells

Project description:We characterized the DNA sequences spanning telomere fusion junctions focusing on XpYp:17p inter-chromosomal events in HCT116 LIG3-/-:mL3, HCT116 LIG4-/-, WT HCT116 and MRC5HPVE6E7 cells.

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