LFQ of indisulam-dependent biotinylated peptides from HCT116 cells expressing AirID-DCAF15

ABSTRACT: HCT116 cells stably expressing AGIA-AirID-DCAF15-WT or -D475N mutant were treated with DMSO or 5 µM indisulam in the presence of 50 µM biotin and 10 µM MG132 for 6 h in three biological replicates. After tryptic digestion, biotinylated peptides were enriched with Tamavidin 2-REV and analyzed by LC-MS/MS.

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Hidetaka Kosako 

PROVIDER: PXD028762 | JPOST Repository | Sat Nov 06 00:00:00 GMT 2021

REPOSITORIES: jPOST