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Spheroid of rhabdomyosarcoma cells cultured with muscle extracts

ABSTRACT: We prepared the muscle extracts using detergent, solubilize them by pepsin digestion, and used them for the spheroid culture of human rhabdomyosarcoma cells using a low-attachment tissue culture plate. In this study, we confirmed that the muscle extracts contained proteins whose expression was unique to muscles. In this project, we prepared the spheroid of rhabdomyosarcoma with muscle extracts with 10% content.

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Tadashi Kondo

PROVIDER: PXD029225 | JPOST Repository | Thu Oct 20 00:00:00 BST 2022

REPOSITORIES: jPOST

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spheroid of rhabdomyosarcoma cells cultured with muscle extracts

Project description:We prepared the muscle extracts using detergent, solubilize them by pepsin digestion, and used them for the spheroid culture of human rhabdomyosarcoma cells using a low-attachment tissue culture plate. In this study, we confirmed that the muscle extracts contained proteins whose expression was unique to muscles. In this project, we prepared the spheroid of rhabdomyosarcoma without muscle extracts as a control.

2022-10-20 | PXD029224 | JPOST Repository

spheroid of rhabdomyosarcoma cells cultured with muscle extracts

Project description:We prepared the muscle extracts using detergent, solubilize them by pepsin digestion, and used them for the spheroid culture of human rhabdomyosarcoma cells using a low-attachment tissue culture plate. In this study, we confirmed that the muscle extracts contained proteins whose expression was unique to muscles. In this study, we prepared the muscle extracts using mouse muscle tissues.

2022-10-20 | PXD029223 | JPOST Repository

Gene expression profile of A549 cells cultured with novel 3D cell culture method

Project description:Preclinical cancer drug discovery efforts have employed two-dimensional (2D)-cell-based assay models, which fail to forecast in vivo efficacy and contribute to a lower success rates of clinical approval. Three-dimensional (3D) cell culture models are recently expected to bridge the gap between 2D and in vivo models. We have developed novel 3D culture method that improves the growth of spheroid-forming cancer cells under anchorage-independent condition by leveraging a feature of FP001. Gene microarrays were used to observe the global gene expression in A549 cells cultured with normal adhesion plate (2D, control) or with low adhesion plate (+FP001) and identified distinct classes of up or down-regulated genes.

2015-07-31 | GSE69477 | GEO

Gene expression profile of A549 cells cultured with FP001

Project description:Preclinical cancer drug discovery efforts have employed two-dimensional (2D)-cell-based assay models, which fail to forecast in vivo efficacy and contribute to a lower success rates of clinical approval. Three-dimensional (3D) cell culture models are recently expected to bridge the gap between 2D and in vivo models. We have developed novel 3D culture method that improves the growth of spheroid-forming cancer cells under anchorage-independent condition by leveraging a feature of FP001. Gene microarrays were used to observe the global gene expression in A549 cells cultured in multi-well plate with or without FP001 and identified distinct classes of up or down-regulated genes. A549 cells were cultured for 5 days in normal attachment plate with normal medium (as control) or normal attachment plate with FP001 containing medium. Each sample was collected three times.

2015-07-31 | E-GEOD-69577 | biostudies-arrayexpress

Gene expression profile of A549 cells cultured with FP001

2015-07-31 | GSE69577 | GEO

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Transcriptome analysis of the differentiated C2C12 cells in 3 different substrate, Tissue culture plate, Methacarylated gelatin and 15% Methacarylated gelatin mixed with 1% PSS (poly sodium 4-styrenesulfonate)

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Expression data from Human primary colon cancer cells

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