Proteomics

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Deep proteome analysis by DIA-MS with FAIMS

ABSTRACT: We aimed to expand the proteome coverage by single-shot measurements using optimizing high-field asymmetric waveform ion mobility spectrometry (FAIMS) parameters in DIA-MS. In addition, we analyzed host proteins in the feces of control and repeated social defeat stress (R-SDS) model mice with optimal FAIMS parameters in DIA-MS.

ORGANISM(S): Homo Sapiens (human) Mus Musculus (mouse)

SUBMITTER: Yusuke Kawashima 

PROVIDER: PXD029853 | JPOST Repository | Thu Apr 28 00:00:00 GMT+01:00 2022

REPOSITORIES: jPOST

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Dataset's files

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Action DRS
60min_oDIA-FAIMS_HEK293_200ng_CV-40V_IT100OT100_1.raw Raw
60min_oDIA-FAIMS_HEK293_200ng_CV-40V_IT100OT100_2.raw Raw
60min_oDIA-FAIMS_HEK293_200ng_CV-40V_IT100OT100_3.raw Raw
60min_oDIA-FAIMS_HEK293_200ng_CV-45V_IT100OT100_1.raw Raw
60min_oDIA-FAIMS_HEK293_200ng_CV-45V_IT100OT100_2.raw Raw
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Publications

Single-Shot 10K Proteome Approach: Over 10,000 Protein Identifications by Data-Independent Acquisition-Based Single-Shot Proteomics with Ion Mobility Spectrometry.

Kawashima Yusuke Y   Nagai Hirotaka H   Konno Ryo R   Ishikawa Masaki M   Nakajima Daisuke D   Sato Hironori H   Nakamura Ren R   Furuyashiki Tomoyuki T   Ohara Osamu O  

Journal of proteome research 20220506 6

The evolution of mass spectrometry (MS) and analytical techniques has led to the demand for proteome analysis with high proteome coverage in single-shot measurements. Focus has been placed on data-independent acquisition (DIA)-MS and ion mobility spectrometry as techniques for deep proteome analysis. We aimed to expand the proteome coverage by single-shot measurements using optimizing high-field asymmetric waveform ion mobility spectrometry parameters in DIA-MS. With our established proteome ana  ...[more]