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TMT-10plex analysis of endogenous proteins expressed in HEK-293 cells as a recombinant transporter assay system

ABSTRACT: Tandem-mass-tags (TMT)-10plex proteomics characterized endogenous proteins expressed in HEK-293 cells transiently transfected with control vector or transporter cDNA. Proteomic profiles between mock (n=3), naïve (n=3), and HEK-293 cells transfected with MATE1 cDNA (1, 10, 30, 100 ng) were compared.

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Yoshikatsu Kanai

PROVIDER: PXD030022 | JPOST Repository | Thu Jul 07 00:00:00 BST 2022

REPOSITORIES: jPOST

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Functional coupling of organic anion transporter OAT10 (SLC22A13) and monocarboxylate transporter MCT1 (SLC16A1) influencing the transport function of OAT10.

Ohtsu Naoko N Ohgaki Ryuichi R Jin Chunhuan C Xu Minhui M Okanishi Hiroki H Takahashi Ryo R Matsui Akiko A Kishimoto Wataru W Ishiguro Naoki N Kanai Yoshikatsu Y

Journal of pharmacological sciences 20220617 1

OAT10 (SLC22A13) is a transporter highly expressed in renal tubules and transporting organic anions including nicotinate, β-hydroxybutyrate, p-aminohippurate, and orotate. In transport assays using Xenopus oocytes and HEK293 cells, we found that apparent substrate selectivity of OAT10 was different between the expression systems, particularly less pronounced uptake of β-hydroxybutyrate in HEK293 cells. Because functional coupling between transporters may interfere with functional properties of t ...[more]

PMID: 35926947

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