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Proteomics of necrotic granulomas in lungs of C3HeB/FeJ mice infected with M. tuberculosis

ABSTRACT: C3heB/FeJ mice were infected with M. tuberculosis to form necrotic granulomatous lesions. FFPE samples of infected lungs with granulomas were microdissected into three distinct regions, Caseum, foamy macrophage, and Cell. Proteins were extracted from microdissected samples, followed by LC-MS/MS.

ORGANISM(S): Mus Musculus (mouse)

SUBMITTER: Shintaro Seto

PROVIDER: PXD030088 | JPOST Repository | Tue Sep 06 00:00:00 BST 2022

REPOSITORIES: jPOST

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Spatial multiomic profiling reveals the novel polarization of foamy macrophages within necrotic granulomatous lesions developed in lungs of C3HeB/FeJ mice infected with Mycobacterium tuberculosis.

Seto Shintaro S Nakamura Hajime H Guo Tz-Chun TC Hikichi Haruka H Wakabayashi Keiko K Miyabayashi Akiko A Nagata Toshi T Hijikata Minako M Keicho Naoto N

Frontiers in cellular and infection microbiology 20220927

Infection with Mycobacterium tuberculosis leads to the development of tuberculosis (TB) with the formation of granulomatous lesions. Foamy macrophages (FM) are a hallmark of TB granulomas, because they provide the primary platform of M. tuberculosis proliferation and the main source of caseous necrosis. In this study, we applied spatial multiomic profiling to identify the signatures of FM within the necrotic granulomas developed in a mouse model resembling human TB histopathology. ...[more]

PMID: 36237431

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