Proteomics

Dataset Information

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Total proteome Dry seed Col-0, dwa1 dwa2, YFP:AFP2 (+/-) and YFP:AFP2 in dwa1 dwa2 Background

ABSTRACT: we performed a label-free quantitative mass spectrometry-based proteomic analysis comparing the dry seed proteome of four genotypes in three independent biological replicates: Col-0, dwa1 dwa2 double mutant, and YFP-AFP2 over expressing lines in both Col-0 and dwa1 dwa2 backgrounds. This assay aimed to identified protein accumulation pattern correlating with seed longevity and or ABA resistance during germination.

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

SUBMITTER: Guillaume Née 

PROVIDER: PXD030589 | JPOST Repository | Wed Dec 21 00:00:00 GMT 2022

REPOSITORIES: jPOST

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Publications

ABI5 binding protein2 inhibits ABA responses during germination without ABA-INSENSITIVE5 degradation.

Lynch Tim T   Née Guillaume G   Chu Avan A   Krüger Thorben T   Finkemeier Iris I   Finkelstein Ruth R RR  

Plant physiology 20220601 2

Overexpression of ABA-INSENSITIVE5 binding proteins (AFPs) results in extreme ABA resistance of seeds and failure to acquire desiccation tolerance, at least in part through effects on chromatin modification. We tested the hypothesis that AFPs promote germination in Arabidopsis (Arabidopsis thaliana) by also functioning as adapters for E3 ligases that ubiquitinate ABI5, leading to its degradation. Interactions between AFPs and two well-characterized classes of E3 ligases targeting ABI5, DWD HYPER  ...[more]