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Brain microvascular endothelial cells: PODXL KD

ABSTRACT: The purpose of the present study was, therefore, to explore the effect of PODXL-knockdown on the protein expression and transport function in the immortalized human BMECs (hCMEC/D3) as a human BBB model. We established PODXL-knockdown hCMEC/D3 cells, and protein expressions were comprehensively analyzed using quantitative proteomics.

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Sumimo Ohtsuki

PROVIDER: PXD030652 | JPOST Repository | Fri Mar 11 00:00:00 GMT 2022

REPOSITORIES: jPOST

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Knockdown of Podocalyxin Post-Transcriptionally Induces the Expression and Activity of ABCB1/MDR1 in Human Brain Microvascular Endothelial Cells.

Nagano Hinako H Ogata Seiryo S Ito Shingo S Masuda Takeshi T Ohtsuki Sumio S

Journal of pharmaceutical sciences 20220217 6

Podocalyxin (PODXL) is a highly sialylated transmembrane protein that is expressed on the luminal membrane of brain microvascular endothelial cells. To clarify the role of PODXL in the blood-brain barrier (BBB), the present study aimed to investigate the effect of PODXL-knockdown on protein expression, especially the expression of ABCB1/MDR1, in human microvascular endothelial cells (hCMEC/D3). By quantitative proteomics, gene ontology enrichment with differentially expressed proteins showed tha ...[more]

PMID: 35182544

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