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Evaluation of suitability of dried saliva spots for in-depth proteome analyses of clinical applications

ABSTRACT: We performed a single-shot DIA-MS proteome analysis of dried saliva spots (DSS) to investigate whether it provides a platform to complement the dried blood spot (DBS) data.

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Hironori Sato

PROVIDER: PXD031181 | JPOST Repository | Tue Apr 26 00:00:00 BST 2022

REPOSITORIES: jPOST

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Evaluation of the Suitability of Dried Saliva Spots for In-Depth Proteome Analyses for Clinical Applications.

Sato Hironori H Nakajima Daisuke D Ishikawa Masaki M Konno Ryo R Nakamura Ren R Ohara Osamu O Kawashima Yusuke Y

Journal of proteome research 20220421 5

Previously, we performed nontargeted proteome analysis using dried blood spots (DBSs) that are widely used in newborn screening for the clinical diagnosis of congenital genetic diseases and immunodeficiency. We have developed an efficient and simple pretreatment method for DBSs that can detect more than 1000 proteins. To complement proteins that are difficult to detect via DBS analysis with less invasive alternative body fluids, we conducted this study to investigate the proteins detected from d ...[more]

PMID: 35446574

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