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SNO protein screening

ABSTRACT: S-Nitrosylated proteins in Saccharomyces cerevisiae were analyzed. S-Nitrosoglutathione was added to S. cerevisiae extract to induce S-nitrosylation. Thereafter, biotinylation was carried out by a biotin switch method, and pulldown was carried out by an avidin resin. After separation by SDS-PAGE, they were digested with trypsin and subjected to LC-MS.

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

SUBMITTER: Shino Seiya

PROVIDER: PXD031724 | JPOST Repository | Fri Feb 17 00:00:00 GMT 2023

REPOSITORIES: jPOST

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