Proteomics

Dataset Information

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Peptides from abortive pep-tRNAs in wt and delta-pth2 strain of S. cerevisiae

ABSTRACT: The peptides obtained from RNA fraction (with TriPURE isolation reagent). Two strains (WT and delta-pth2), and three technical replicates for each strain.

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

SUBMITTER: Hideki Taguchi 

PROVIDER: PXD031958 | JPOST Repository | Tue Dec 06 00:00:00 GMT 2022

REPOSITORIES: jPOST

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Publications

Nascent peptide-induced translation discontinuation in eukaryotes impacts biased amino acid usage in proteomes.

Ito Yosuke Y   Chadani Yuhei Y   Niwa Tatsuya T   Yamakawa Ayako A   Machida Kodai K   Imataka Hiroaki H   Taguchi Hideki H  

Nature communications 20221202 1

Robust translation elongation of any given amino acid sequence is required to shape proteomes. Nevertheless, nascent peptides occasionally destabilize ribosomes, since consecutive negatively charged residues in bacterial nascent chains can stochastically induce discontinuation of translation, in a phenomenon termed intrinsic ribosome destabilization (IRD). Here, using budding yeast and a human factor-based reconstituted translation system, we show that IRD also occurs in eukaryotic translation.  ...[more]