Dataset Information

Mitochondrial alternative NADH dehydrogenases limit ROS, protect chloroplasts and promote survival of reoxygenation in Arabidopsis

ABSTRACT: Weather extremes are increasing worldwide frequently exposing plants to transient flooding. When floodwaters recede, plants have to cope with increased light intensity and elevated oxygen that favor excessive formation of reactive oxygen species (ROS). We report a novel molecular mechanism that helps Arabidopsis leaves cope with reoxygenation in the light that reveals a central role of the alternative electron tranport in mitochodria in cellular ROS management that is beneficial for photosynthetic recovery and provides tolerance to reoxygenation stress.

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

SUBMITTER: MARGRET SAUTER

PROVIDER: PXD032119 | JPOST Repository | Fri Dec 02 00:00:00 GMT 2022

REPOSITORIES: jPOST

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Publications

Mitochondrial alternative NADH dehydrogenases NDA1 and NDA2 promote survival of reoxygenation stress in Arabidopsis by safeguarding photosynthesis and limiting ROS generation.

Jethva Jay J Lichtenauer Sophie S Schmidt-Schippers Romy R Steffen-Heins Anja A Poschet Gernot G Wirtz Markus M van Dongen Joost T JT Eirich Jürgen J Finkemeier Iris I Bilger Wolfgang W Schwarzländer Markus M Sauter Margret M

The New phytologist 20230204 1

Plant submergence stress is a growing problem for global agriculture. During desubmergence, rising O<sub>2</sub> concentrations meet a highly reduced mitochondrial electron transport chain (mETC) in the cells. This combination favors the generation of reactive oxygen species (ROS) by the mitochondria, which at excess can cause damage. The cellular mechanisms underpinning the management of reoxygenation stress are not fully understood. We investigated the role of alternative NADH dehydrogenases ( ...[more]

PMID: 36464787

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Project description:Global gene expression was compared between roots of cotton plants (variety Sicot 71) flooded for 4 hours and roots of unflooded cotton plants. Global gene expression was also compared between leaves of cotton plants (variety Sicot 71) flooded for 24 hours and leaves of unflooded cotton plants. Waterlogging stress causes yield reductions in cotton (Gossypium hirsutum L.). A major component of waterlogging stress is the lack of oxygen available to submerged tissues. While changes in expressed protein, gene transcription and metabolite levels have been studied in response to low oxygen stress, little research has been done on molecular responses to waterlogging in cotton. We assessed cotton growth responses to waterlogging and assayed global gene transcription responses in root and leaf cotton tissues of partially submerged plants. Waterlogging causes significant reductions in stem elongation, shoot mass, root mass, and leaf number. At the global gene expression level waterlogging significantly alters the expression of 1012 genes (4.2% of genes assayed) in root tissue as early as 4h after flooding. Many of these genes are associated with cell wall modification and growth pathways, glycolysis, fermentation, mitochondrial electron transport and nitrogen metabolism. Waterlogging of plant roots also altered global leaf gene expression, significantly changing the expression of 1305 genes (5.4% of genes assayed) after 24h of flooding. Genes associated with cell wall growth and modification, tetrapyrrole synthesis, hormone response, starch metabolism and nitrogen metabolism were affected in leaf tissues of waterlogged plants. Implications of these results for the development of waterlogging tolerant cotton are discussed. Keywords: Stress Response Plants of cotton cultivar Sicot 71 were grown to the two-leaf stage in tubs. For stress treatments plants were either watered as normal or flooded with water to completely submerge the root system. At four and twenty-four hours post-flooding samples of root or leaf tissue were taken from control and flooded plants. Total RNA was extracted from each tissue sample and assayed on cotton Affymetrix chips. Two biological replications were used for each comparison.

Dataset Information

Mitochondrial alternative NADH dehydrogenases limit ROS, protect chloroplasts and promote survival of reoxygenation in Arabidopsis

Publications

Mitochondrial alternative NADH dehydrogenases NDA1 and NDA2 promote survival of reoxygenation stress in Arabidopsis by safeguarding photosynthesis and limiting ROS generation.

Similar Datasets

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