Proteomics

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Identification of CLPX binding proteins in FT293 cells

ABSTRACT: FT293 cells which express FLAG-tagged CLPX, in which deleted endogenous CLPX were used for the study. FT 293 cells which express a chimeric protein composed of the mitochondrial transit peptide region of ornithine transcarbamylase and Flag-tagged luciferase were used as control. Each of FLAG-tagged proteins were expressed by doxycycline-inducible manner. The FLAG-tagged proteins were immunoprecipitated using anti-DDDK-tag antibody-conjugated agarose beads, and the immunoprecipitated proteins were eluted from the agarose beads by DDDDK peptides. The eluted precipitates were digested with trypsin.

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Ko Suzuki 

PROVIDER: PXD032346 | JPOST Repository | Thu Mar 17 00:00:00 GMT 2022

REPOSITORIES: jPOST

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CLPX regulates mitochondrial fatty acid β-oxidation in liver cells.

Suzuki Ko K   Kubota Yoshiko Y   Kaneko Kiriko K   Kamata Costantine Chasama CC   Furuyama Kazumichi K  

The Journal of biological chemistry 20230903 10

Mitochondrial fatty acid oxidation (β-oxidation) is an essential metabolic process for energy production in eukaryotic cells, but the regulatory mechanisms of this pathway are largely unknown. In the present study, we found that several enzymes involved in β-oxidation are associated with CLPX, the AAA+ unfoldase that is a component of the mitochondrial matrix protease ClpXP. The suppression of CLPX expression increased β-oxidation activity in the HepG2 cell line and in primary human hepatocytes  ...[more]

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