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Impact of P-glycoprotein on intracellular drug concentration in peripheral blood mononuclear cells and K562 cells

ABSTRACT: P-gp protein expression levels were evaluated in PBMC and lymphocyte with or without induction using TMT-10 plex procedure

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Takuma Katayama

PROVIDER: PXD033283 | JPOST Repository | Tue Apr 18 00:00:00 BST 2023

REPOSITORIES: jPOST

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Impact of P-glycoprotein on intracellular drug concentration in peripheral blood mononuclear cells and K562 cells.

Ito Kohei K Naoi Marina M Nishiyama Kotaro K Kudo Takashi T Tsuda Yasuhiro Y MacLean Caroline C Ishiguro Naoki N

Drug metabolism and pharmacokinetics 20221216

P-glycoprotein (P-gp) expression in lymphocytes is variable and 2-fold higher in rheumatoid arthritis (RA) patients with treatment resistance than in healthy subjects. To date the information on P-gp-mediated drug interaction in lymphocyte is limited. We analyzed the importance on P-gp in lymphocytes using peripheral blood mononuclear cells (PBMCs) together with K562, K562/Adr, and K562/Vin cells, which have various P-gp levels, as cell models, and dexamethasone, nintedanib and apafant as weak t ...[more]

PMID: 36724603

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