Proteomics

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Development of parallel reaction monitoring mass spectrometry assay for the detection of human norovirus major capsid protein

ABSTRACT: Human Norwalk virus (HuNoVs), the most common etiological agents of acute gastroenteritis, are genetically diverse RNA viruses that frequently cause mass food poisoning internationally. In this study, we developed a parallel reaction monitoring (PRM) assay to simultaneously detect and identify representative peptides derived from VP1 in six different HuNoV genotypes using a high-resolution mass spectrometer. An optimized protocol was also used to detect and quantify VP1 protein in human stool specimens. This method is expected to become a new tool for studying the molecular epidemiology of HuNoV and to shed new light on targeted proteomics in clinical practice.

ORGANISM(S): Homo Sapiens (human) Norwalk Virus

SUBMITTER: Akihide Ryo 

PROVIDER: PXD033981 | JPOST Repository | Tue Feb 21 00:00:00 GMT 2023

REPOSITORIES: jPOST

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Development of Parallel Reaction Monitoring Mass Spectrometry Assay for the Detection of Human Norovirus Major Capsid Protein.

Kimura Yayoi Y   Shin Jihye J   Nakai Yusuke Y   Takahashi Masaya M   Ino Yoko Y   Akiyama Tomoko T   Goto Keiko K   Nagata Noriko N   Yamaoka Yutaro Y   Miyakawa Kei K   Kimura Hirokazu H   Ryo Akihide A  

Viruses 20220628 7

Human Norwalk viruses (HuNoVs), the most common etiological agents of acute gastroenteritis, are genetically diverse RNA viruses that frequently cause mass food poisoning internationally. Although nucleic acid detection methods, such as reverse transcription-quantitative polymerase chain reaction (RT-qPCR), are the gold standard for the diagnosis of norovirus infection, alternative methods are needed for the specific and sensitive viral protein detection for rapid diagnosis and surveillance. In  ...[more]