Project description:The goal of this study was to compare the transcriptome profile (RNA-seq) of perinatal hearts of cardiomyocyte-specific RXRab-deficient (edKO) and WT mice, to identify genes that are controlled by the expression of the TF RXRa and RXRb.
Project description:The goal of this study was to compare the epigenetic landscape of perinatal hearts of cardiomyocyte-specific RXRab-deficient (edKO) and WT mice, to identify differential chromatin dynamics that are controlled by TF RXRa and RXRb.
Project description:The goal of this study was to compare the cistrome profile of H3K27ac (ChIP-seq) of perinatal hearts of cardiomyocyte-specific RXRab-deficient and WT mice, in order to identify genomic regions that are differentially activated by TF RXRa and RXRb.
Project description:The goal of this study was to compare the cistrome profile of RXR (ChIP-seq) of perinatal hearts of cardiomyocyte-specific RXRab-deficient and WT mice, in order to identify genomic regions which are directly controlled by TF RXRa and RXRb.
Project description:We found that cardiomyocyte-specific PRMT1-deficient (PRMT1-cKO) mice showed dilated cardiomyopathy and aberrant cardiac alternative splicing. To identify novel cardiac splicing events, we performed a comprehensive analysis of gene expression changes in hearts of wildtype (WT) and PRMT1-cKO mice using RNA sequencing (RNA-Seq). To investigate differentially expressed genes (false discovery rate (FDR) p<0.05, fold change >2) between control and PRMT1-cKO mice, we performed pairwise comparisons of RNA-Seq data using the CLC Genomics Workbench software.
Project description:Mice are on a C57Bl/6J background strain, hearts were collected from Clock and WT mice at ZT07, following 24 weeks on either a HF or SC diet. The microarray approach allows the investigation of gene expression changes of all genes in Clock HFD vs. Clock SC vs. WT HFD vs. WT SC hearts.
Project description:The RNA-sequence analysis of cardiomyocyte-specific deletion of STAT3 mice hearts showed that comparing with WT mice hearts, the STAT3cKO mice hearts showed reduced cardiac function by affecting some key pathways.
Project description:LRRK2 mutations are associated with both familial and sporadic forms of Parkinson’s disease (PD). Convergent evidence suggests that LRRK2 plays critical roles in regulating striatal function. Here, by using knock-in mouse lines that express the two most common LRRK2 pathogenic mutations—G2019S and R1441C—we investigated how pathogenic LRRK2 mutations altered striatal physiology. To identify the signaling pathways that underlie the motor learning deficits, specifically in the RC mice we performed six-plex tandem mass tag (TMT) quantitative mass spectrometry (MS) to compare the protein expression of either RC or GS and WT mice following the five days of the rotarod test.
Project description:Role of B cells against pulmonary infection caused by Acinetobacter baumannii is poorly understood. In this study we performed RNA-seq of whole lung tissue to compare the gene expression profile of WT C57BL/6 and B cell deficient mice (muMT) at 4 hours post infection (4hpi). We also compared the gene expression profile of naive WT and muMT mice. Here we report that naive WT mice had significantly higher expression of numerous genes related to antimicrobial peptide production, leukocyte chemotaxis and activation. We also found that the B cell deficiency lead to higher expression of genes associated with decreased pulmonary respiratory rate, increased pulmonary ventillation rate, and increased recruitment of pulmonary eosinophils at 4hpi. This observation suggests a higher pulmonary infammatory response in B cell deficient mice. Taken together, this study expands our current understanding about the role of B cells in controlling A. baumannii at the early stages of respiratory infection.