Proteomics

Dataset Information

0

Ultra-fast proteome analysis of HEK293T cells using Q-Orbitrap MS

ABSTRACT: We established an ultrafast proteomic method based on a 5-min gradient LC and quadrupole-Orbitrap mass spectrometer.

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Yusuke Kawashima 

PROVIDER: PXD034227 | JPOST Repository | Thu Jul 28 00:00:00 BST 2022

REPOSITORIES: jPOST

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Publications

Optimization of Ultrafast Proteomics Using an LC-Quadrupole-Orbitrap Mass Spectrometer with Data-Independent Acquisition.

Ishikawa Masaki M   Konno Ryo R   Nakajima Daisuke D   Gotoh Mari M   Fukasawa Keiko K   Sato Hironori H   Nakamura Ren R   Ohara Osamu O   Kawashima Yusuke Y  

Journal of proteome research 20220801 9

Proteomics has become an increasingly important tool in medical and medicinal applications. It is necessary to improve the analytical throughput for these applications, particularly in large-scale drug screening to enable measurement of a large number of samples. In this study, we aimed to establish an ultrafast proteomic method based on 5-min gradient LC and quadrupole-Orbitrap mass spectrometer (Q-Orbitrap MS). We precisely optimized data-independent acquisition (DIA) parameters for 5-min grad  ...[more]