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Proteomic analysis of the mouse cerebral cortex in ketamine-induced schizophrenia

ABSTRACT: The aim of this proteomic analysis was to identify potential biomarkers involved in the ketamine-induced schizophrenia. Eight-week-old male ICR mice were either intraperitoneally injected with ketamine (5 mg/kg/day) or saline for 7 days. Than, the brains were analyzed by FD-LC-MS/MS proteomic method.

ORGANISM(S): Mus Musculus (mouse)

SUBMITTER: Jen-Ai Lee

PROVIDER: PXD036846 | JPOST Repository | Mon Sep 19 00:00:00 BST 2022

REPOSITORIES: jPOST

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Project description:Scope: As a result of population ageing, the number of Alzheimer’s disease (AD) patients has rapidly increased. There are many hypothesises on the pathogenesis of AD, but its detailed molecular mechanism is still unknown, and so no effective preventive or therapeutic measures have been established. Some reports showed a decrease in levels of norepinephrine (NE) has been suspected to be involved in the decline of cognitive function in AD patients and NE concentrations were decreased in postmortem AD patient brains. Tyr-Trp was identified as being the most effective dipeptide in enhancing norepinephrine (NE) synthesis and metabolism. And Tyr-Trp treatment ameliorated the short-term memory dysfunction in AD model mice caused by amyloid beta (Aβ) 25-35. So, the purpose of this study was to investigate the preventive or/and protective effects of Tyr-Trp administration in AD model mice. Methods and results: ddY mice were fed normal diet. After 7 days of feeding, mice were divided into 3 groups (n=10 per group) as follows: the sham group, which received saline administration and distilled water injection; the Aβ group, which received saline administration and Aβ peptides 25–35 injection; and the Aβ+YW group, which received Tyr-Trp administration and Aβ peptides 25–35 injection. Tyr-Trp was orally administered Tyr-Trp (100 mg kg-1 day-1, twice a day), starting 7 days before Aβ peptides injection. The other groups received oral administration of saline (5 mL kg-1). After Aβ peptides injection or sham operation, each groups received every oral administration. DAN microarray showed that Tyrosine hydroxylase, dopa decarboxylase and dopamine receptor D2 mRNA expressions which were downregulated in Aβ group comparing with sham group were upregulated in Aβ+YW group comparing with Aβ group. In addition, quinoid dihydropteridine reductase mRNA expression which wasn’t changed in Aβ group comparing with sham group was upregulated in Aβ+YW group comparing with Aβ group. Conclusions: These results suggest that Tyr-Trp administration might ameliorate the short-term memory dysfunction in AD model mice because of enhancing norepinephrine (NE) synthesis and metabolism through metabolism of both Tyr and Trp.

			Action	DRS
	20170221_1_1.xps	Other
	20170221_1_13.xps	Other
	20170221_1_15.xps	Other
	20170221_1_16.xps	Other
	20170221_1_17.xps	Other

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Proteomic analysis of the mouse cerebral cortex in ketamine-induced schizophrenia

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