Proteomics

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Generation and utilization of a monoclonal antibody against hepatitis B virus core protein for a comprehensive interactome analysis

ABSTRACT: Hepatitis B virus (HBV) core antigen (HBc) is a structural protein that forms the viral nucleocapsid and is involved in various steps of the viral replication cycle, but its role in the pathogenesis of HBV infection is still elusive. In this study, we generated a mouse monoclonal antibody against HBc and used it in an antibody-based in situ biotinylation to identify the host proteins that interact with HBc.

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Akihide Ryo 

PROVIDER: PXD036961 | JPOST Repository | Tue Feb 21 00:00:00 GMT 2023

REPOSITORIES: jPOST

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Generation and Utilization of a Monoclonal Antibody against Hepatitis B Virus Core Protein for a Comprehensive Interactome Analysis.

Nakai Yusuke Y   Miyakawa Kei K   Yamaoka Yutaro Y   Hatayama Yasuyoshi Y   Nishi Mayuko M   Suzuki Hidefumi H   Kimura Hirokazu H   Takahashi Hidehisa H   Kimura Yayoi Y   Ryo Akihide A  

Microorganisms 20221130 12

Hepatitis B virus (HBV) core antigen (HBc) is a structural protein that forms the viral nucleocapsid and is involved in various steps of the viral replication cycle, but its role in the pathogenesis of HBV infection is still elusive. In this study, we generated a mouse monoclonal antibody (mAb) against HBc and used it in antibody-based in situ biotinylation analysis in order to identify host proteins that interact with HBc. HBc antigen was produced with a wheat germ cell-free protein synthesis s  ...[more]