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Mouse brain perivascular astrocyte endfoot proteome

ABSTRACT: Proteome analysis of isolated mouse brain blood vessels and perivascular astrocyte endfeet (PV-AEF) using DIA.

ORGANISM(S): Mus Musculus (mouse)

SUBMITTER: Prof. Yoshimi Takai

PROVIDER: PXD037949 | JPOST Repository | Sat Nov 04 00:00:00 GMT 2023

REPOSITORIES: jPOST

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Heterogeneity of perivascular astrocyte endfeet depending on vascular regions in the mouse brain.

Kameyama Takeshi T Miyata Muneaki M Shiotani Hajime H Adachi Jun J Kakuta Soichiro S Uchiyama Yasuo Y Mizutani Kiyohito K Takai Yoshimi Y

iScience 20230921 10

Astrocytes interact with not only synapses but also brain blood vessels through perivascular astrocyte endfeet (PV-AEF) to form the neurovascular unit (NVU). However, PV-AEF components have not been fully identified. Here, we biochemically isolated blood vessels from mouse brain homogenates and purified PV-AEF. The purified PV-AEF were observed in different sizes, similar to PV-AEF on brain blood vessels. Mass spectrometry analysis identified 9,762 proteins in the purified PV-AEF, including cell ...[more]

PMID: 37829206

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