Dataset Information

MS-based determination of NELF-A phosphorylation

ABSTRACT: LC-MS/MS analysis of Flag-NELF-A purified from TPA-stimulated HEK293 cells

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Masaaki Oyama

PROVIDER: PXD038094 | JPOST Repository | Tue Nov 15 00:00:00 GMT 2022

REPOSITORIES: jPOST

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Dataset's files

Source:

			Action	DRS
	140605_hata_140605_oe_02_refseq_human8_P_CarbamidoM_PRS.xlsx	Xlsx
	140605_oe_02.raw	Raw

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Publications

ERK-mediated NELF-A phosphorylation promotes transcription elongation of immediate-early genes by releasing promoter-proximal pausing of RNA polymerase II.

Ohe Seina S Kubota Yuji Y Yamaguchi Kiyoshi K Takagi Yusuke Y Nashimoto Junichiro J Kozuka-Hata Hiroko H Oyama Masaaki M Furukawa Yoichi Y Takekawa Mutsuhiro M

Nature communications 20221203 1

Growth factor-induced, ERK-mediated induction of immediate-early genes (IEGs) is crucial for cell growth and tumorigenesis. Although IEG expression is mainly regulated at the level of transcription elongation by RNA polymerase-II (Pol-II) promoter-proximal pausing and its release, the role of ERK in this process remains unknown. Here, we identified negative elongation factor (NELF)-A as an ERK substrate. Upon growth factor stimulation, ERK phosphorylates NELF-A, which dissociates NELF from pause ...[more]

PMID: 36463234

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