Proteomics

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TMT-based quantitative proteomic analysis of SAHA-treated human HeLa cells

ABSTRACT: We performed a TMT-based quantitative proteomic analysis of SAHA-perturbed human HeLa cells using advanced RTS platform on Orbitrap Eclipse Tribrid MS system.

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Masaaki Oyama 

PROVIDER: PXD038681 | JPOST Repository | Fri Jun 09 00:00:00 BST 2023

REPOSITORIES: jPOST

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Publications

Real-Time Search-Assisted Multiplexed Quantitative Proteomics Reveals System-Wide Translational Regulation of Non-Canonical Short Open Reading Frames.

Kozuka-Hata Hiroko H   Hiroki Tomoko T   Miyamura Naoaki N   Kitamura Aya A   Tsumoto Kouhei K   Inoue Jun-Ichiro JI   Oyama Masaaki M  

Biomolecules 20230612 6

Abnormal expression of histone deacetylases (HDACs) is reported to be associated with angiogenesis, metastasis and chemotherapy resistance regarding cancer in a wide range of previous studies. Suberoylanilide hydroxamic acid (SAHA) is well known to function as a pan-inhibitor for HDACs and recognized as one of the therapeutic drug candidates to epigenetically coordinate cancer cell fate regulation on a genomic scale. Here, we established a Real-Time Search (RTS)-assisted mass spectrometric platf  ...[more]

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