Pyrophosphoproteomics on NOLC1 / TCOF1 / IWS1 pulldown samples

ABSTRACT: Known pyrophosphorylation substrates NOLC1, TCOF1, IWS1 were overexpressed in IP6K1-/- double KO HEK293T cells, along with active WT or kinase-dead IP6K1 i.e. background high or low in inositol pyrophosphates. the three proteins were pulled down via their SFB-tag and submitted to a pyrophosphoproteomics workflow. This was to demonstrate the dependence of pyrophosphorylation on PP-InsPs.

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Dorothea Fiedler, Rashna Bhandari 

PROVIDER: PXD038962 | JPOST Repository | Tue Mar 12 00:00:00 GMT 2024

REPOSITORIES: jPOST