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Proteome analysis by simple and stable nanoESI spray system

ABSTRACT: We developed InSpIon system capable of stable nanoESI spray. Here, K562 cell digests were measured by DDA-MS and DIA-MS with the typical open nanoESI spray system (Open system) and the spray system inserted spray tip head into ion transfer tube (Insertion-ITT system), and the InSpIon system.

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Yusuke Kawashima

PROVIDER: PXD039557 | JPOST Repository | Wed Apr 05 00:00:00 BST 2023

REPOSITORIES: jPOST

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Dataset's files

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			Action	DRS
	InSpIon_0800v_DDA-20min_K562-20ng.raw	Raw
	InSpIon_1800v_DDA-20min_K562-20ng.raw	Raw
	InSpIon_2000v_DDA-20min_K562-20ng.raw	Raw
	InSpIon_2200v_DDA-20min_K562-20ng.raw	Raw
	Insertion-ITT_0800v_DDA-20min_K562-20ng.raw	Raw

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Publications

Development of a Simple and Stable NanoESI Spray System Using Suction Wind from the MS Inlet.

Kawashima Yusuke Y Ishikawa Masaki M Konno Ryo R Nakajima Daisuke D Ohara Osamu O

Journal of proteome research 20230410 5

Improving the reproducibility of proteome analysis systems presents a challenge; therefore, in this study, we developed a new insertion spray ionization (InSpIon) system wherein an InSpIon tube was designed with a spray tip inserted into the tube. This system stabilized the spray and subsequently improved the reproducibility of the analysis. ...[more]

PMID: 37036810

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