Dataset Information

Identification of phosphorylation sites in human recombinant TOP2B

ABSTRACT: Human recombinant full-length TOP2B purified from HEK293 cells were phosphorylated with Erk1 and Erk2 in vitro. After in-gel digestion, the phosphorylation sites were determined using mass spectrometry.

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Heeyoun Bunch

PROVIDER: PXD040977 | JPOST Repository | Fri Nov 03 00:00:00 GMT 2023

REPOSITORIES: jPOST

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Dataset's files

Source:

			Action	DRS
	SN221362_deTOP2B_control_1ul.mgf	Mgf
	SN221362_deTOP2B_control_1ul.mzid	Mzid
	SN221362_deTOP2B_control_1ul.raw	Raw
	SN221363_deTOP2B_Erk1_1ul.mgf	Mgf
	SN221363_deTOP2B_Erk1_1ul.mzid	Mzid

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Publications

ERK2-topoisomerase II regulatory axis is important for gene activation in immediate early genes.

Bunch Heeyoun H Kim Deukyeong D Naganuma Masahiro M Nakagawa Reiko R Cong Anh A Jeong Jaehyeon J Ehara Haruhiko H Vu Hongha H Chang Jeong Ho JH Schellenberg Matthew J MJ Sekine Shun-Ichi SI

Nature communications 20231214 1

The function of the mitogen-activated protein kinase signaling pathway is required for the activation of immediate early genes (IEGs), including EGR1 and FOS, for cell growth and proliferation. Recent studies have identified topoisomerase II (TOP2) as one of the important regulators of the transcriptional activation of IEGs. However, the mechanism underlying transcriptional regulation involving TOP2 in IEG activation has remained unknown. Here, we demonstrate that ERK2, but not ERK1, is importan ...[more]

PMID: 38097570

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