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Mapping adipocyte interactome networks by HaloTag-enrichment–mass spectrometry

ABSTRACT: Mapping protein interaction complexes in their natural state in a cell in vivo is a holy grail of protein network analysis. We here describe the development of HaloMS, a high-throughput HaloTag-based affinity purification–mass spectrometry assay for protein interaction discovery. The approach enabled quick capture of newly expressed protein and eliminated the tedious conventional one-by-one assay. As a proof-of-principle, we used HaloMS to evaluate protein complex interactions of 17 regulatory proteins in human adipocyte. The adipocyte interactome network was validated using an in vitro pull-down assay and artificial intelligence-based prediction tools. The application of HaloMS to probe adipocyte differentiation signaling pathways allowed the identification of previously unknown transcription factor–protein complexes and revealed proteome-wide human adipocyte transcription factor networks. This study using new technology reveals hidden networks of adipocyte signaling pathways, shedding light on how signals from different pathways are integrated.

ORGANISM(S): Homo Sapiens (human) Arabidopsis Thaliana (mouse-ear Cress)

SUBMITTER: Junshi Yazaki

PROVIDER: PXD041085 | JPOST Repository | Wed May 22 00:00:00 BST 2024

REPOSITORIES: jPOST

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			Action	DRS
	PK21022-01_A6.raw	Raw
	PK21022-02_B6.raw	Raw
	PK21022-03_C6.raw	Raw
	PK21022-04_D6.raw	Raw
	PK21022-05_E6.raw	Raw

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