Proteomics

Dataset Information

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The original mouse bone spectral library for the DIA-MS

ABSTRACT: We successfully constructed a customized spectral library containing information on 1,394 proteins for DIA-MS; the library can be used for comprehensive identification and quantification of proteins expressed in the mouse bones.

ORGANISM(S): Mus Musculus (mouse)

SUBMITTER: Yayoi Kimura 

PROVIDER: PXD041731 | JPOST Repository | Tue Aug 01 00:00:00 BST 2023

REPOSITORIES: jPOST

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Publications

Identification of gravity-responsive proteins in the femur of spaceflight mice using a quantitative proteomic approach.

Egashira Kenji K   Ino Yoko Y   Nakai Yusuke Y   Ohira Takashi T   Akiyama Tomoko T   Moriyama Kayano K   Yamamoto Yu Y   Kimura Mitsuo M   Ryo Akihide A   Saito Tomoyuki T   Inaba Yutaka Y   Hirano Hisashi H   Kumagai Ken K   Kimura Yayoi Y  

Journal of proteomics 20230722

Although the microgravity (μ-g) environment that astronauts encounter during spaceflight can cause severe acute bone loss, the molecular mechanism of this bone loss remains unclear. To investigate the gravity-response proteins involved in bone metabolism, it is important to comprehensively determine which proteins exhibit differential abundance associated with mechanical stimuli. However, comprehensive proteomic analysis using small bone samples is difficult because protein extraction in mineral  ...[more]

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