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Proteomic analysis of FK506 treated cancer cells

ABSTRACT: Hs578T cells were treated with FK506 for 3h and 12h, and proteomics analysis performed to identify proteins affected by FK506 treatment

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Midori Shimada

PROVIDER: PXD042833 | JPOST Repository | Mon Dec 30 00:00:00 GMT 2024

REPOSITORIES: jPOST

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Dataset's files

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			Action	DRS
	001_Control_500ng_T120_DDA_B4.wiff	Wiff
	001_Control_500ng_T120_DDA_B4.wiff.scan	Wiff
	001_Control_500ng_T120_SW_B4.wiff	Wiff
	001_Control_500ng_T120_SW_B4.wiff.scan	Wiff
	002_Fkbp_3h_500ng_T120_DDA_B4.wiff	Wiff

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Publications

Calcineurin/NFATc1 pathway represses cellular cytotoxicity by modulating histone H3 expression.

Sato Yuki Y Habara Makoto M Hanaki Shunsuke S Sharif Jafar J Tomiyasu Haruki H Miki Yosei Y Shimada Midori M

Scientific reports 20240626 1

Excess amounts of histones in the cell induce mitotic chromosome loss and genomic instability, and are therefore detrimental to cell survival. In yeast, excess histones are degraded by the proteasome mediated via the DNA damage response factor Rad53. Histone expression, therefore, is tightly regulated at the protein level. Our understanding of the transcriptional regulation of histone genes is far from complete. In this study, we found that calcineurin inhibitor treatment increased histone prote ...[more]

PMID: 38926604

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Transcriptomic, (phospho)proteomic, and metabolomic analysis of tumor-comprising cells treated by photodynamic therapy [mouse]

Project description:Photodynamic therapy (PDT) is a tumor treatment strategy that relies on the production of reactive oxygen species (ROS) in the tumor following local illumination. Although PDT has shown promising results in the treatment of non-resectable perihilar cholangiocarcinoma, it is still employed palliatively. In this study, tumor-comprising cells (i.e., cancer cells, endothelial cells, macrophages) were treated with the photosensitizer zinc phthalocyanine that was encapsulated in cationic liposomes (ZPCLs). Post-PDT survival pathways were studied following sublethal (50% lethal concentration (LC50)) and supralethal (LC90) PDT using a multi-omics approach. ZPCLs did not exhibit toxicity in any of the cells as assessed by toxicogenomics. Sublethal PDT induced survival signaling in perihilar cholangiocarcinoma (SK-ChA-1) cells via mainly hypoxia-inducible factor 1 (HIF-1)-, nuclear factor of kappa light polypeptide gene enhancer in B cells (NF-кB)-, activator protein 1 (AP-1)-, and heat shock factor (HSF)-mediated pathways. In contrast, supralethal PDT damage was associated with a dampened survival response. (Phospho)proteomic and metabolomic analysis showed that PDT-subjected SK-ChA-1 cells downregulated proteins associated with epidermal growth factor receptor (EGFR) signaling, particularly at LC50. PDT also affected various components of glycolysis and the tricarboxylic acid cycle as well as metabolites involved in redox signaling. In conclusion, sublethal PDT activates multiple pathways in tumor parenchymal and non-parenchymal cells that, in tumor cells, transcriptionally regulate cell survival, proliferation, energy metabolism, detoxification, inflammation/angiogenesis, and metastasis. Accordingly, sublethally afflicted tumor cells are a major therapeutic culprit. Our multi-omics analysis unveiled multiple druggable targets for pharmacological intervention.

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