TurboID-mediated proximal labeling of Drosophila caspase Drice in adult brains

ABSTRACT: V5-TurboID was knock-in tagged in Drosophila caspase Drice which is expressed in adult brains. Flies were fed with 100 µM biotin for protein biotinylation in two biological replicates. Biotinylated protein-containing lysate extracted from adult brains was subjected to FG-NeutrAvidin beads purification followed by on-beads trypsin digestion. Digested peptides were desalinated and purified using GL-tip SDB. Purified peptides were subjected to LC-MS/MS analysis.

ORGANISM(S): Drosophila Melanogaster (fruit Fly)

SUBMITTER: Masayuki Miura 

PROVIDER: PXD042922 | JPOST Repository | Tue Feb 06 00:00:00 GMT 2024

REPOSITORIES: jPOST