Proteomics

Dataset Information

0

TurboID-mediated proximal labeling of Drosophila caspase Drice in adult brains


ABSTRACT: V5-TurboID was knock-in tagged in Drosophila caspase Drice which is expressed in adult brains. Flies were fed with 100 µM biotin for protein biotinylation in two biological replicates. Biotinylated protein-containing lysate extracted from adult brains was subjected to FG-NeutrAvidin beads purification followed by on-beads trypsin digestion. Digested peptides were desalinated and purified using GL-tip SDB. Purified peptides were subjected to LC-MS/MS analysis.

ORGANISM(S): Drosophila Melanogaster (fruit Fly)

SUBMITTER: Masayuki Miura 

PROVIDER: PXD042922 | JPOST Repository | Tue Feb 06 00:00:00 GMT 2024

REPOSITORIES: jPOST

Dataset's files

Source:
Action DRS
20210215Hanawa03.raw Raw
20210215Hanawa05.raw Raw
20210215Hanawa07.raw Raw
20210215Hanawa09.raw Raw
20210215_DriceV5TurboID_normalized.pdResult Other
Items per page:
1 - 5 of 6
altmetric image

Publications

Executioner caspase is proximal to Fasciclin 3 which facilitates non-lethal activation in <i>Drosophila</i> olfactory receptor neurons.

Muramoto Masaya M   Hanawa Nozomi N   Okumura Misako M   Chihara Takahiro T   Miura Masayuki M   Shinoda Natsuki N  

eLife 20250617


The nervous system undergoes functional modification independent of cell turnover. Caspase participates in reversible neuronal modulation via non-lethal activation. However, the mechanism that enables non-lethal activation remains unclear. Here, we analyzed proximal proteins of <i>Drosophila</i> executioner caspase in the adult brain using TurboID. We discovered that executioner caspase Drice is, as an inactive proform, proximal to cell membrane proteins, including a specific splicing isoform of  ...[more]

Similar Datasets

2023-05-11 | PXD033797 | JPOST Repository
| PRJNA807666 | ENA
2022-04-21 | GSE196884 | GEO
2023-07-10 | GSE155565 | GEO
2025-08-20 | PXD055017 | JPOST Repository
2025-06-11 | PXD054313 | JPOST Repository
2023-03-01 | GSE154686 | GEO
2022-02-16 | PXD023520 | Pride
| PRJNA650308 | ENA
2025-02-20 | GSE167544 | GEO