Unbiased phosphoproteomics analysis of human myotubes unveils extramitotic CDK1 kinase activity modulates insulin signaling

ABSTRACT: We performed total proteome and phosphoproteome analysis to characterize the role of non-mitotic kinase activity of CDK1 in insulin signaling through pharmacological inhibition and translational repression via siRNA-based knockdown. Specifically, cultured myotubes were treated with RO-3306, a selective ATP competitive inhibitor to inhibit CDK1 activity, or transfected with a CDK1 targeting siRNA pool to decrease CDK1 expression.

ORGANISM(S): Mus Musculus (mouse)

SUBMITTER: E Shyong Tai 

PROVIDER: PXD043414 | JPOST Repository | Thu Jul 04 00:00:00 BST 2024

REPOSITORIES: jPOST