Proteomics

Dataset Information

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Interactome of DYRK2 by using BioID proximity labeling

ABSTRACT: In this study, DYRK2 was found to be an important regulator of Hedgehog signaling. To identify DYRK2 interacting proteins, we performed interactome using BioID proximity labeling. NIH3T3 cells containing Dox-inducible DYRK2-BioID2-HA construct were cultured in the media with or without Doxycycline for DYRK2-BioID2-HA expression, followed by proximity labeling. The lysates from Doxycycline induction (DYRK2) and without induction (Mock) were subjected to streptavidin pull-down and the proteins were identified by LC-MS/MS.

ORGANISM(S): Mus Musculus (mouse)

SUBMITTER: Shushi Nagamori 

PROVIDER: PXD043445 | JPOST Repository | Mon Jul 01 00:00:00 BST 2024

REPOSITORIES: jPOST

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Dataset's files

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Action DRS
BioID2-DYRK2%20PD2.5.xlsx Xlsx
DYRK2_F1.raw Raw
DYRK2_F2.raw Raw
DYRK2_F3.raw Raw
mock_F1.raw Raw
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Publications

Positive regulation of Hedgehog signaling via phosphorylation of GLI2/GLI3 by DYRK2 kinase.

Yoshida Saishu S   Kawamura Akira A   Aoki Katsuhiko K   Wiriyasermkul Pattama P   Sugimoto Shinya S   Tomiyoshi Junnosuke J   Tajima Ayasa A   Ishida Yamato Y   Katoh Yohei Y   Tsukada Takehiro T   Tsuneoka Yousuke Y   Yamada Kohji K   Nagamori Shushi S   Nakayama Kazuhisa K   Yoshida Kiyotsugu K  

Proceedings of the National Academy of Sciences of the United States of America 20240705 28

Hedgehog (Hh) signaling, an evolutionarily conserved pathway, plays an essential role in development and tumorigenesis, making it a promising drug target. Multiple negative regulators are known to govern Hh signaling; however, how activated Smoothened (SMO) participates in the activation of downstream GLI2 and GLI3 remains unclear. Herein, we identified the ciliary kinase DYRK2 as a positive regulator of the GLI2 and GLI3 transcription factors for Hh signaling. Transcriptome and interactome anal  ...[more]

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