Interactome of DYRK2 by using BioID proximity labeling

ABSTRACT: In this study, DYRK2 was found to be an important regulator of Hedgehog signaling. To identify DYRK2 interacting proteins, we performed interactome using BioID proximity labeling. NIH3T3 cells containing Dox-inducible DYRK2-BioID2-HA construct were cultured in the media with or without Doxycycline for DYRK2-BioID2-HA expression, followed by proximity labeling. The lysates from Doxycycline induction (DYRK2) and without induction (Mock) were subjected to streptavidin pull-down and the proteins were identified by LC-MS/MS.

ORGANISM(S): Mus Musculus (mouse)

SUBMITTER: Shushi Nagamori 

PROVIDER: PXD043445 | JPOST Repository | Mon Jul 01 00:00:00 BST 2024

REPOSITORIES: jPOST