Dataset Information

Top-Down native MS/MS crosslinked alpha synuclein with ion mobility and ECD

ABSTRACT: Recombinant human alpha synuclein crosslinked with 2.5 molar excess BS2G and BS3 crosslinkers. Buffer exchanged into 50 mM ammonium acetate. MS/MS on singly crosslinked protein by top-down ECD. Final protein concentration 50 uM. Positive mode static nanospray.

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Ian Webb

PROVIDER: PXD043876 | JPOST Repository | Tue Jul 18 00:00:00 BST 2023

REPOSITORIES: jPOST

ACCESS DATA

Similar Datasets

Project description:Abnormal accumulation of aggregated proteins and sustained microglial activation are important contributors of neurodegenerative process in neurological diseases. Recent studies have shown that aggregation-prone proteins, such as a-synuclein, the protein implicated in Parkinson’s disease (PD), are released from neuronal cells and thus present in the extracellular fluid, pointing to the possible paracrine effects of these proteins on microglial immune responses. However, the mechanism underlying the disease-associated microglial activation and the role of neuronal proteins in this process remain unknown. Here, we show that extracellular a-synuclein released from neuronal cells is an endogenous ligand of toll-like receptor 2 (TLR2) and activates microglia, which in turn induces neurodegeneration. Interaction between neuron-released a-synuclein and TLR2 and subsequent activation of the TLR2 signaling were demonstrated comprehensively by using computational modeling of signaling network and by the experimental validation in TLR2-deficient microglia both in vitro and in vivo. In contrast to the neuron-released a-synuclein, recombinant a-synuclein proteins, including monomer, oligomer, fibril, or nitrated forms, were not able to interact or activate TLR2, suggesting that neuronal cells have a mechanism of enriching specific forms of a-synuclein capable of activating TLR2 during the process of releasing this protein. Taken together, the results suggest that both neuron-released extracellular a-synuclein and TLR2 might be novel therapeutic targets for modifying neuroinflammation in PD and related neurodegenerative diseases. We collected culture media from differentiated SH-SY5Y cells overexpressing either human a-synuclein (alpha-SCM) or beta-galactosidase (LZCM) and treat these media to primary rat microglia at the concentration of a-synuclein of 1.1M. Transcriptome analyses with microglial cells treated with either aSCM or LZCM at two different time points, 6 h and 24 h.

Dataset Information

Top-Down native MS/MS crosslinked alpha synuclein with ion mobility and ECD

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure