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MS data on ubiquitinated proteins

ABSTRACT: Mass spectrometric analysis of ubiquitinated proteins in MDS-L cells

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Masaaki Oyama

PROVIDER: PXD044347 | JPOST Repository | Fri Mar 08 00:00:00 GMT 2024

REPOSITORIES: jPOST

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			Action	DRS
	20211224_kaito_MDS_L_WT.raw	Raw
	20211228_kaito_MDS_L_KO.raw	Raw
	20220215_hata_20211224_kaito_MDS_L_WT.xlsx	Xlsx
	20220215_hata_20211228_kaito_MDS_L_KO.xlsx	Xlsx

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Publications

Inhibition of TOPORS ubiquitin ligase augments the efficacy of DNA hypomethylating agents through DNMT1 stabilization.

Kaito Satoshi S Aoyama Kazumasa K Oshima Motohiko M Tsuchiya Akiho A Miyota Makiko M Yamashita Masayuki M Koide Shuhei S Nakajima-Takagi Yaeko Y Kozuka-Hata Hiroko H Oyama Masaaki M Yogo Takao T Yabushita Tomohiro T Ito Ryoji R Ueno Masaya M Hirao Atsushi A Tohyama Kaoru K Li Chao C Kawabata Kimihito Cojin KC Yamaguchi Kiyoshi K Furukawa Yoichi Y Kosako Hidetaka H Yoshimi Akihide A Goyama Susumu S Nannya Yasuhito Y Ogawa Seishi S Agger Karl K Helin Kristian K Yamazaki Satoshi S Koseki Haruhiko H Doki Noriko N Harada Yuka Y Harada Hironori H Nishiyama Atsuya A Nakanishi Makoto M Iwama Atsushi A

Nature communications 20240828 1

DNA hypomethylating agents (HMAs) are used for the treatment of myeloid malignancies, although their therapeutic effects have been unsatisfactory. Here we show that CRISPR-Cas9 screening reveals that knockout of topoisomerase 1-binding arginine/serine-rich protein (TOPORS), which encodes a ubiquitin/SUMO E3 ligase, augments the efficacy of HMAs on myeloid leukemic cells with little effect on normal hematopoiesis, suggesting that TOPORS is involved in resistance to HMAs. HMAs are incorporated int ...[more]

PMID: 39198387

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