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Low-input proteomics using lauryl maltose neopentyl glycol

ABSTRACT: We developed a sample preparation method for low-input proteomics using lauryl maltose neopentyl glycol. Using the developed method, we performed proteome analysis of 100ng of HEK293T proteins, coIP samples, and serum extracellular vesicles, and further challenged label-free single-cell proteomics of HEK293F cells.

ORGANISM(S): Homo Sapiens (human) Bos Taurus

SUBMITTER: Yusuke Kawashima

PROVIDER: PXD044605 | JPOST Repository | Wed Mar 06 00:00:00 GMT 2024

REPOSITORIES: jPOST

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Universal Pretreatment Development for Low-input Proteomics Using Lauryl Maltose Neopentyl Glycol.

Konno Ryo R Ishikawa Masaki M Nakajima Daisuke D Endo Yusuke Y Ohara Osamu O Kawashima Yusuke Y

Molecular & cellular proteomics : MCP 20240304 4

In recent years, there has been a growing demand for low-input proteomics, particularly in the context of single-cell proteomics (SCP). In this study, we have developed a lauryl maltose neopentyl glycol (LMNG)-assisted sample preparation (LASP) method. This method effectively reduces protein and peptide loss in samples by incorporating LMNG, a surfactant, into the digestion solution and subsequently removing the LMNG simply via reversed phase solid-phase extraction. The advantage of removing LMN ...[more]

PMID: 38447790

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