Dataset Information

Identification of GDPP in cancer secretome

ABSTRACT: Prostate cancer cell lines were cultured with serum-free media, for 48 h. The cultured media were collected and lyophilized. The lyophilized media were dissolved in ammonium bicarbonate containing 4 M urea, and proteins were de-salted by acetone-precipitation and subjected to proteome analysis.

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Noriaki Arakawa

PROVIDER: PXD045369 | JPOST Repository | Wed Jan 01 00:00:00 GMT 2025

REPOSITORIES: jPOST

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Dataset's files

Source:

			Action	DRS
	121102-Urology-13%20(LNCaP_2).raw	Raw
	121102-Urology-13%20(LNCaP_2)_mgf.mgf	Mgf
	121102-Urology-19%20(Du145_2).raw	Raw
	121102-Urology-19%20(Du145_2)_mgf.mgf	Mgf
	121102-Urology-20%20(PC3_2).raw	Raw

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Publications

GDF15 propeptide promotes bone metastasis of castration-resistant prostate cancer by augmenting the bone microenvironment.

Yamamichi Gaku G Kato Taigo T Arakawa Noriaki N Ino Yoko Y Ujike Takeshi T Nakano Kosuke K Koh Yoko Y Motoyama Yuichi Y Outani Hidetatsu H Myoba Shohei S Ishizuya Yu Y Yamamoto Yoshiyuki Y Hatano Koji K Kawashima Atsunari A Fukuhara Shinichiro S Uemura Hiroji H Okada Seiji S Morii Eiichi E Nonomura Norio N Uemura Motohide M

Biomarker research 20241125 1

<h4>Background</h4>Bone metastasis (BM) is a common and fatal condition in patients with castration-resistant prostate cancer (CRPC). However, there are no useful blood biomarkers for CRPC with BM, and the mechanism underlying BM is unclear. In this study, we investigated precise blood biomarkers for evaluating BM that can improve the prognosis of patients with CRPC.<h4>Methods</h4>We comprehensively examined culture supernatants from four prostate cancer (PCa) cell lines using Orbitrap mass spe ...[more]

PMID: 39587633

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Project description:Plant nutrition takes advantage by the simultaneous presence of more N forms in the rhizosphere. In the last decades the interplay between ammonium and nitrate acquisition systems in roots has been deeply investigated. Although widely used as fertilizers, the occurrence of cross connection between urea and ammonium nutrition has been scarcely studied in plants, especially at molecular level. In a recent paper we provided evidence that maize plants fed with urea and ammonium mix showed a better N-uptake efficiency than plants fed with ammonium or urea alone. To elucidate the molecular mechanism underlying this response, transcriptomic and metabolomic changes occurring in maize plants were investigated. Transcriptomic analyses indicated that several transporters and enzymes involved in N-nutrition were found upregulated by all three N-treatments (AMT1.3, NRT1.1, NRT2.1, GS1, GOGAT, GDH), confirming that urea is a direct source of N for plants. Depending on N-form available in nutrient solution a peculiar response at transcriptomic and metabolomic level was observed, especially after 24 hours of treatment. In comparison to one single N-form, urea and ammonium mix induced a prompt assimilation of N, characterized by an overaccumulation of main amino acids in shoots, and an upregulation of ZmAMT1.1. Moreover even a peculiar modulation of aquaporins, carbonic anydrases, glutamine synthetase, amino aspartate, as well as the glycolysis-TCA cycle was induced in roots by urea and ammonium mix. Depending on N-form available in the external media, even changes in phytohormone’s composition were observed in maize (CKs, ABA, JA); in particular, already after 24 hours of treatment, urea induced the accumulation of trans-zeatin in shoots. Through a multiomics approach, we provide for the first time molecular characterization of maize response to urea and ammonium nutrition. This study paves the way to formulate guidelines for the optimization of N fertilization of crops to improve the N use efficiency in plants and therefore limit N losses in the environment.