Dataset Information

Identification of interacting proteins with toxoplasma gondii GRA25

ABSTRACT: Identification of interacting proteins with toxoplasma gondii GRA25

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Takumi Koshiba

PROVIDER: PXD046935 | JPOST Repository | Thu Nov 14 00:00:00 GMT 2024

REPOSITORIES: jPOST

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Dataset's files

Source:

			Action	DRS
	200610_01HEK_ctrl1_Slot1-09_1_621.d.zip	Other
	200610_02HEK_ctrl2_Slot1-09_1_622.d.zip	Other
	200610_03HEK_GRA1_Slot1-10_1_624.d.zip	Other
	200610_04HEK_GRA2_Slot1-10_1_625.d.zip	Other
	BioID_GRA25_PeptideQuant.csv	Csv

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Project description:Toxoplasma gondii is an obligate intracellular parasite which can cause toxoplasmosis. Surface and secreted proteins of T. gondii play important roles in infection and immunity, and also are antigen targets in immunological diagnosis and vaccine development. However, it is difficult to investigate identities and antigenicities of surface and secreted proteins due to limitation of surface protein extraction methods. In this study, a total of 785 potential surface and secreted proteins of T. gondii RH tachyzoites were identified using a method combination of biotin labeling, avidin chromatography isolation, and high flux proteomics (LC-MS/MS). Among the highly-expressed 65 proteins, 43 proteins (66%) were originally annotated as surface or secreted proteins in the released T. gondii genomes, which proved the method combination to be a credible strategy. Furthermore, the transcriptomic responses and cytokine secretions induced by the isolated proteins, the live T. gondii RH tachyzoites infection, and the live pathogenic E. coli infection, in the human peripheral blood monocyte THP-1 cell lines, were comparatively analyzed to reveal antigenicities and immunobiological properties of T. gondii surface and secreted proteins. The transciptomic profiles induced by the isolated proteins were similar to those induced by the live bacterium infection, but were different from those induced by the live parasite infection. Contrary to the low cytokine secretion induced by the live parasite infection, the isolated proteins induced significant cytokine and chemokines secretion. Especially, the secretions of several chemokines induced by the isolated proteins were even higher than those induced by the live bacterium infection. These data suggested that T. gondii surface and secreted proteins were effective antigens, while the live parasite could evade the host innate immunity. This study comprehensively revealed the identities and antigenicities of T. gondii surface and secreted proteins, which laid foundation for further screening new T. gondii antigens, developing immunological diagnosis methods, and studying host immune response to T. gondii infection.

Dataset Information

Identification of interacting proteins with toxoplasma gondii GRA25

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