Project description:Obesity is a significant risk factor for diabetes, cardiovascular diseases, and certain cancers, and manifests as excessive fat accumulation. The browning of white adipose tissue (WAT) represents one of the most promising strategies for preventing and treating obesity and metabolic diseases. To date, an increasing number of studies have focused on key molecular mechanisms regulating fat thermogenesis, laying the foundation for effective intervention strategies.We analyzed the differentially expressed proteins in white adipose tissue of the inguinal region using mass spectrometry.
Project description:MThe methionine reactivity in subcutaneous white adipose tissue (sWAT) samples from the non-tumor or tumor mice was unbiasedly analyzed using oxaziridine chemical tool. It provides which proteinaceous methinoines in sWAT could regulated under the progression of cachexia by pancreatic ductal adenocarcinoma (PDAC).
Project description:The aim of this study was to identify genes expressed selectively in brown adipose tissue as compared to white adipose tissue from the same animals. This analysis provides a gene set that is brown and white adipose selective. Keywords: tissue comparison from mice
Project description:Analysis of visceral white adipose tissue (EWAT) from Yin Yang 1 adipose-specific knockout mice exposed to cold (4ºC) for 4 days. Control mice YY1flox/flox versus YY1flox/flox; Adiponectin Cre were cold exposed for 4 days.
Project description:White adipose tissue is a central place to energy storage and a major endocrine organ. However, adipose molecular mechanisms have been poorly studied during prolonged fasting. To fill this gap, the aim of this study was to decipher proteomic regulations in rat adipose tissue during phase 2 (lipid mobilization) and phase 3 (protein catabolism) of prolonged fasting compared to the fed state. Specific responses reflecting adipose tissue inflammation, increased fibrinolysis and a possible protein catabolism-related energy saving mechanism were recorded during phase 3. Differences between internal and subcutaneous adipose tissues were essentially related to lipid metabolism, the response to oxidative stress and energy production. These data thus provide a molecular basis of adipose tissue responses according to the fasting stage.
Project description:Using high throughput sequencing we report 5hmC levels, and gene expression changes in mouse Inguinal white adipose tissue upon exposure to room temperature and 7 days to cold in Tet1 floxed wild-type control and adipose selectiveTet1 KO mice.
Project description:Bulk RNA-sequencing of 27 C57BL/6 gonadal white adipose tissue samples where ovariectomized females were gavaged 4 days for 92 days with 0.01 to 30 µg/kg TCDD or sesame oil vehicle (n = 3) per dose.
Project description:The aim of this study was to identify genes expressed selectively in brown adipose tissue as compared to white adipose tissue from the same animals. This analysis provides a gene set that is brown and white adipose selective. Experiment Overall Design: Interscapular brown adipose tissue and epididymal white adipose tissue was carefully dissected from 3 male C57Bl/6 mice. These samples were profiled independently using Affymetrix mouse 430_2 gene arrays, representing 3 biological replicates for each brown and white adipose tissues.