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Proteome analysis of serum proteins enriched by STL, LEL and WGA

ABSTRACT: Serum proteins enriched by solanum tuberosum lectin (STL), lycopersicon esculentum lectin (LEL) and wheat germ agglutinin (WGA) were analyzed by LC-DIA-MS/MS using Q-Exactive HF-X (ThermoFisher Scientific).

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Yusuke Kawashima

PROVIDER: PXD048327 | JPOST Repository | Sat Feb 24 00:00:00 GMT 2024

REPOSITORIES: jPOST

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Proteome Analysis of Serum Purified Using <i>Solanum tuberosum</i> and <i>Lycopersicon esculentum</i> Lectins.

Nakajima Daisuke D Konno Ryo R Miyashita Yasuomi Y Ishikawa Masaki M Ohara Osamu O Kawashima Yusuke Y

International journal of molecular sciences 20240121 2

Serum and plasma exhibit a broad dynamic range of protein concentrations, posing challenges for proteome analysis. Various technologies have been developed to reduce this complexity, including high-abundance depletion methods utilizing antibody columns, extracellular vesicle enrichment techniques, and trace protein enrichment using nanobead cocktails. Here, we employed lectins to address this, thereby extending the scope of biomarker discovery in serum or plasma using a novel approach. We enrich ...[more]

PMID: 38279312

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