Proteomics

Dataset Information

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Mass Spectometry analysis of the DnaK clients


ABSTRACT: A co-affinity assay coupled to downstream LC-MS analyses were used to investigate the effect of linker mutations on the DnaK interactome as previously described3. The purified recombinant forms of DnaK and DnaKLS were immobilized (as bait) by His-tag affinity on HisPur cobalt resin suspended in TBS (10 mM Tris-HCl pH 7.5, 50 mM NaCl and 10 Mm imidazole) and incubated at 4 °C for 4 hours with gently rocking motion to allow maximum binding.

ORGANISM(S): Escherichia Coli

SUBMITTER: Tawanda Zininga 

PROVIDER: PXD049037 | JPOST Repository | Fri Mar 28 00:00:00 GMT 2025

REPOSITORIES: jPOST

Dataset's files

Source:
Action DRS
201911_TZ_DMK_UB1_Node_27.mgf Mgf
201911_TZ_DMK_UB2.raw Raw
201911_TZ_DMK_UB2_Node_27.mgf Mgf
201911_TZ_D_Node_27.mgf Mgf
201911_TZ_KJM_109_2.raw Raw
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