Proteomics

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Nuclear and total proteins in THP-1 cell differentiation


ABSTRACT: THP-1 monocyte-like suspension cells can be differentiated into macrophage-like adherent cells by TPA (PMA) treatment. Proteomic analysis was carried out on (i) normal THP-1 cells, (ii) THP-1 cells in which KPNA1 (importin-alpha5) was knocked down or not by siRNA, and (iii) THP-1 TetOn-KPNA2 (importin-alpha1) cells induced or not by Dox. Total and nuclear proteins were prepared from both the monocyte-like and macrophage-like cells and quantified by a label-free method.

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Makoto Kimura 

PROVIDER: PXD049401 | JPOST Repository | Sat Feb 15 00:00:00 GMT 2025

REPOSITORIES: jPOST

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