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Identification of neuronal surface autoantigens in serum samples from a patient with thymoma-associated limbic encephalitis, comparison of pre- and postoperative thymoma by LC-MS/MS analysis

ABSTRACT: Mouse brain tissue lysates were immunoprecipitated with protein A/G Sepharose beads which had been incubated with serum from patient or control participant. The immune complexes were subsequently separated by SDS‒PAGE. Bands were excised from the gel, and trypsin-digested samples were subjected to liquid chromatography-tandem mass spectrometry (LC-MS/MS) using an Orbitrap Velos Pro (Thermo Scientific). MS/MS dataset analysis was performed using the Mascot software program (Matrix Science).

ORGANISM(S): Mus Musculus (mouse)

SUBMITTER: Tomoaki Tanaka

PROVIDER: PXD052274 | JPOST Repository | Fri May 16 00:00:00 BST 2025

REPOSITORIES: jPOST

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			Action	DRS
	orb_170125_OO-TT-22_%2310.raw	Raw
	orb_170125_OO-TT-22_%2311.raw	Raw
	orb_170125_OO-TT-22_%2312.raw	Raw
	orb_170125_OO-TT-22_%2313.raw	Raw
	orb_170125_OO-TT-22_%2314.raw	Raw

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Identification of neuronal surface autoantigens in serum samples from a patient with thymoma-associated limbic encephalitis, comparison of pre- and postoperative thymoma by LC-MS/MS analysis

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