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Single-Cell Secretome Profiling by NanoLC-MS/MS Analysis

ABSTRACT: Here, we reported a novel single cell secretome profiling method that integrates cell metabolic labeling, on-capillary enrichment based on click chemistry, in-situ sample preparation, and nanoLC-MS/MS analysis. By this method, an average of circa 389 protein groups were confidently identified as the secretome from single HeLa cells, an order of magnitude higher than previously reported targeted secretome analysis by antibody recognition.

ORGANISM(S): Homo Sapiens (human) Cellular Organisms

SUBMITTER: Lihua Zhang

PROVIDER: PXD054112 | JPOST Repository | Fri Nov 07 00:00:00 GMT 2025

REPOSITORIES: jPOST

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			Action	DRS
	HeLa_1-1-1_SC_01.raw	Raw
	HeLa_1-2-5_SC_02.raw	Raw
	HeLa_1-3-1_SC_03.raw	Raw
	HeLa_2-1-3_SC_04.raw	Raw
	HeLa_2-2-2_SC_05.raw	Raw

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